Wednesday, August 9, 2017

Updated Method to get YDNA haplogroup from AncestryDNA results

Back in 2016 I posted an article explaining how to get YDNA information from AncestryDNA results. While this turned out to be a popular subject with the genetic genealogy community, it was by no means an easy method to follow. Since then a few developments have made the process much simpler. AncestryDNA has updated their testing chip to v5, which more specifically targets SNPs that are used in ethnicity profiles, and more importantly Chris Morley of MorleyDNA.com has updated his YDNA predictor tool to work directly with AncestryDNA test files.  As you'll see below, the method is now much easier to use. 

Note: If you have trouble accessing the MorleyDNA.com website, there are a few alternatives you can try. In my prior article on this subject, I talked about using promethease.com health reports to get haplogroup information.  Another alternative worth trying is wegene.com, a Chinese genomics company that offers free autosomal transfers.  However, wegene.com only provides Y haplogroup information for AncestryDNA tests taken since 2017.

Getting a YDNA Haplogroup from AncestryDNA Results


AncestryDNA has a lot of hidden data within their tests. For men, this includes Y-DNA results, which are partially tested on the AncestryDNA chip, but not used by Ancestry in their current DNA analysis.  However, there is often enough data to make a rough prediction as to a male's Y-DNA haplogroup. This guide explains how to use an online tool to quickly estimate the Y-DNA haplogroup using AncestryDNA results.  (Note: This also works for 23andMe and MyHeritage results).

 Step One: Download your raw data.


 In order to view and use the YDNA data within your AncestryDNA results, you'll first need to download the raw data. Instructions on how to do so can be found in this AncestryDNA Support page. Note the email with the download link described in step 6 can take up to 24 hours to arrive, so be patient. If you don't receive the email within 24 hours, try checking your spam filter, requesting it again, and if that fails, contact Ancestry Support.

The end result will be a zip file downloaded to your computer with a name like "dna-data-2017-8-9.zip" (or whatever date you downloaded the file).

Step Two: Upload the data to MorleyDNA



Go to https://ytree.morleydna.com/extractFromAutosomal and click the "Choose File" button and select the file you downloaded in Step One. There is no need to unzip the compressed zip file.  The page will run a script to parse the YDNA data within the file. This may take a few minutes depending on your computer speed.  Because this is process is performed on your computer, I would not suggest trying this on a mobile device.

Step Three: Confirm the Upload & Submit the Data


You'll next be presented with a summary of the data extracted from the file.  Confirm that it says "no errors to report" in the last box.  If it says something else, you might want to redownload your zip file as it may be corrupted.  Click Submit.  You'll be taken to one more confirmation page. Click "Feed this Data into the MorleyDNA.com Y-SNP Subclade Predictor"

You'll then be presented with another screen of data.  On this screen click the "I'm not a robot" captcha and click Predict. You then will be taken to your results.

Step Four: Interpreting your results. 


You should now see a page that looks something like this:



On the left is a list of potential haplogroups.  On the right are the specific SNPs tested.  Green are SNPs you matched that indicate you may belong on a particular branch.  Red are SNPs that were tested but you did not match the desired mutation.  Essentially you want to look for the deepest branch with the most green and least red.  In the example above, the testee is clearly on the E branch, but not on E1a, as he has a Red negative result there.  Scrolling down I was able to see his correct terminal branch was likely E1b as predicted on the left side of the page.  But in some cases you may have to select the second or third choice to find your correct haplogroup.  It will be the one with the most green in it.  If its a group with only one or two green or a mix of green and multiple red, this is likely not the correct haplogroup.  Also, you should IGNORE root ancestral groups such as BT or F, as these are not present in modern populations.  If BT or F is your most likely group, proceed to the next one on the list with the most green. 

This method is still not perfect.  You are not likely to get a prediction of your ultimate subgroup, as this typically requires testing many more SNPs not covered by the AncestryDNA test. However, for certain haplogroups, this can provide insight into which branch of the Y-DNA tree contains your terminal subgroup.  If your surname is one with a Project at FTDNA, try searching the results for tests with a similar haplogroup and common ancestors to yours.  An FTDNA project adminstrator may be able to help you with additional information, if you can provide them with these results and your paternal ancestry line. And feel free to ask questions in the comments below.

31 comments:

  1. Does this work for women as well as men?

    ReplyDelete
    Replies
    1. It didn't work for me. It told me that my predicted yDNA haplogroup was E-PF1796, but I already know that my paternal yDNA is R1b (R-M343). Luckily, one of my brother's had our paternal line tested.

      Delete
    2. This comment has been removed by the author.

      Delete
    3. I;m all woman, but somehow it predicted my exact known Y haplogroup from my dad's side.

      Delete
  2. I can't imagine this works for women as we don't have the Y chromosome.

    ReplyDelete
  3. I think you meant v2 for AncestryDNA; 23andMe is on v5 now (since some time in August).

    One thing that makes me wonder about the reliability of AncestryDNA markers is that females get some derived results. Those could be picking up XY SNPs.

    ReplyDelete
    Replies
    1. I got My Ancestry DNA results in August and Promethease said I was Male! I freaked out! Seriously thought I had some serious medical issue. I re-downloaded My Raw DNA Data and seen it shows the V2 now and had been regenerated September 2nd. I had fixed the original to V2 just to get it to upload to FTDNA. Ancestry has poor Quality Control. But at least they finally caught it.

      Delete
  4. Replies
    1. I'm replying to my own comment with the fix. The file must be compressed (*.zip) format for the tool to recognize it. Fix came from the International Society of Genetic Genealogy (ISOGG) Facebook group.

      Delete
    2. I had to extract the .zip to get it to work for me.

      Delete
  5. This sounds terrific if I could get it to work for a male member. But it's extremely frustrating that there are do many little things that don't work. And I agree that Ancestry could be more helpful. The biggest these top three get the more competition to deal with.

    ReplyDelete
  6. Replies
    1. I added a note about other sites you can try while MorleyDNA is down. Try wegene.com or promethease.com. Both provide some YDNA haplogroup info from autosomal tests, though they are not as easy to use as Morley's site.

      Delete
  7. what happened to the site? it not there anynore

    ReplyDelete
  8. Thanks. Any way to predicate Mitochondrial haplogroup from Ancestry DNA results?

    ReplyDelete
    Replies
    1. No, I looked into it and there is no mito dna data in ancestrydna results.

      Delete
    2. AncestryDNA does include some mtDNA data, it's under chromosome 26. Not all of the SNPs are particularly relevant for haplogroup designation, though.

      Delete
  9. This comment has been removed by the author.

    ReplyDelete
  10. I have tried to acces the Morley DNA page but it gives error. Doesn't work on Windows 7 pc, and also not on an Android phone.. please help..thank you
    Chris

    ReplyDelete
    Replies
    1. It is working or me on Win10 and Android. I use Chrome. Did you use an Ancestry test? I'm not sure if others like MyHeritage have YDNA data. I don't think it does.

      Delete
  11. I just uploaded my dad's DNA to MorleyDNA. As Promethease showed, it appears he is in Haplogroup R. Now, R alone had 11 green markers. R1b1a had 30 green markers but 1 red marker. Does that one red marker completely eliminate that group? I'm sorry if that's a dumb question. I'm very new to this. This has been the most helpful website I've come across yet.

    ReplyDelete
    Replies
    1. Good question Melody! The answer is no, it does not eliminate that group. I know it is quite common to see one or two red markers. If you don't have the defining SNPs then it is likely negative. Otherwise just having one of the various associated SNPs be negative is not fatal at all. The defining ones are listed first. For R1b1a these are PF6438, L407, RPF6506.

      Delete
  12. This comment has been removed by the author.

    ReplyDelete
  13. At the bottom of the page of the link, it says you use their site at your own risk. So if you give them your DNA profile and they frame you for a crime or clone and replace you, that's just how it is.

    ReplyDelete
  14. Hi, I ran my AncestryDNA through this predictor (ISOGG Tree) and got the following:

    R1b1a2a1a [L11/PF6539/S127], L52/PF6541, L151/PF6542, [PF6546/P310/S129], [P311/S128]

    Here the square brackets [] indicate the dark green highlight.

    I have been 'googling' furiously and can't seem to find anything specifically related to L11, L52 or L151, or to the other name groups PF6539,6541,6542,6546 or P310 or S127,128,129.

    Can anyone offer any insight as to where I can read more about these groups? TIA.

    ReplyDelete
    Replies
    1. I have the same problem. Looks like commonality in our lines
      R1b1a2a1a [L11/PF6539/S127], L151/PF6542, YSC0000082, YSC0000191, L52/PF6541, [PF6546/P310/S129]

      Delete
  15. Does anyone know how a female can find a haplogroup? Seems like all the fun stuff is only available for men. :(

    ReplyDelete
    Replies
    1. AncestryDNA doesn't test mitochondrial DNA, which determines the matrilineal haplogroup. It does have some X DNA markers, which are preferentially inherited thorough females, but that's tricky to analyze since it recombines. But you might check out LivingDNA's test which includes Y DNA, autosomal, and mitochondrial, if you want to have more fun!

      Delete
  16. Has anyone compared all the Y SNPs from AncestryDNA for two different males to see if it can predict their relatedness without focusing on just the terminal haplogroup? I was thinking that folks who share the same patrilineal ancestor should share almost all of the Y SNPs (aside from the various no-calls).

    ReplyDelete